HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV16	E8^E2	GGCTTTGGTATGGGTCGCGG	CGG	R	421	440	65	54.64
2	HPV16	E8^E2	GCATTTAACAGCTCACACAA	AGG	F	244	263	40	48.36
3	HPV18	E8^E2	ATAGAGATATATCATCCACC	TGG	F	356	375	35	62.68
4	HPV18	E8^E2	GGTGCTGGAATACGGTGAGG	GGG	R	406	425	60	59.3
5	HPV18	E8^E2	TGCACCTGTCCAATGCCAGG	TGG	R	124	143	60	47.59
6	HPV18	E8^E2	GATAGTGTACAAATATTGGT	GGG	F	478	497	30	45.03
7	HPV18	E8^E2	GGTGTAGCTGCACCGAGAAG	TGG	R	284	303	60	44.63
8	HPV18	E8^E2	GTATTCCAGCACCGTGTCCG	TGG	F	99	118	60	43.47
9	HPV18	E8^E2	CTACAGGCAACAACAAAAGA	CGG	F	233	252	40	41.86
10	HPV26	E8^E2	GGTGTACTATAAACACAGTT	TGG	R	225	244	35	60.11
11	HPV26	E8^E2	CGTGGGCACCAAGGAAACCG	AGG	F	126	145	65	55.03
12	HPV26	E8^E2	GGAAAGCGACGACGACTCAG	TGG	F	160	179	60	52.81
13	HPV26	E8^E2	GTAGGCTTCGGTGGTCTGGG	TGG	R	445	464	65	49.29
14	HPV26	E8^E2	CTACGTGCCCGTGGGCACCA	AGG	F	117	136	70	47.35
15	HPV26	E8^E2	GGCTTCGGTGGTCTGGGTGG	TGG	R	448	467	70	45.99
16	HPV31	E8^E2	GTTGTTGCGAGGCGACTCCG	TGG	F	216	235	65	54.6
17	HPV31	E8^E2	GGTTTTGGAATTCGATGTGG	TGG	R	439	458	45	50.99
18	HPV31	E8^E2	GCACCAGTGAAGGTGTGCGG	CGG	F	128	147	65	49.94
19	HPV33	E8^E2	ATAGTTCTATGTCATCCACC	TGG	F	338	357	40	60.92
20	HPV33	E8^E2	GTCACTGGTCCAATGCCAGG	TGG	R	127	146	60	56.11
21	HPV33	E8^E2	CTAACTGCACAAACAAGCAG	CGG	F	215	234	45	53.82
22	HPV35	E8^E2	GCGAGTGCGACTCAGTGCCG	TGG	F	198	217	70	59.24
23	HPV35	E8^E2	CCATACCAAAGCCTGCTCCG	TGG	F	102	121	60	51.51
24	HPV35	E8^E2	GGCTTTGGTATGGGTCTCGG	TGG	R	424	443	60	50.19
25	HPV35	E8^E2	TTACACAACAGAATATCAAA	GGG	F	447	466	25	47.33
26	HPV39	E8^E2	GGACACTCCGTCGGGCTCAG	TGG	R	322	341	70	51.73
27	HPV39	E8^E2	GGAAGTGGAAACAGCTGAAG	TGG	F	11	30	50	49.38
28	HPV39	E8^E2	GGTAAGGGAACCAAAAACGC	TGG	F	403	422	50	49.01
29	HPV39	E8^E2	GTACAGGCCACAACACAAGA	CGG	F	245	264	50	47.44
30	HPV39	E8^E2	ACAGAGCCCACTGAGCCCGA	CGG	F	181	200	65	44.6
31	HPV39	E8^E2	GGATTGTTTTTTGGGTGCAT	GGG	R	390	409	40	42.89
32	HPV39	E8^E2	GGTGCATGGGGTTGTTGCGG	TGG	R	403	422	65	41.48
33	HPV45	E8^E2	GGACTCACAGAGCAGCACCA	CGG	F	175	194	60	78.02
34	HPV45	E8^E2	ACAACCTGTCCAATGCCAGG	TGG	R	121	140	55	61.82
35	HPV45	E8^E2	GTACAAGTAACAACAAAAGA	AGG	F	239	258	30	52.34
36	HPV45	E8^E2	CGTGGGTGTTGACACGTCCG	TGG	R	306	325	65	47.3
37	HPV45	E8^E2	TGTTACTTGTACTTGAACAC	AGG	R	270	289	35	42.16
38	HPV51	E8^E2	GGAAGTGGAAGCACTAACAC	TGG	F	238	257	50	58.58
39	HPV51	E8^E2	ATGTGCACTGTCAAACACAA	TGG	R	76	95	40	52.96
40	HPV51	E8^E2	GGACAGTGGGGAGATTGTGG	AGG	R	313	332	60	45.6
41	HPV51	E8^E2	ACAAACAACCAAATACACTG	TGG	F	217	236	35	44.58
42	HPV51	E8^E2	CACAATCTCCCCACTGTCCG	TGG	F	189	208	60	41.12
43	HPV51	E8^E2	TTTGTATTGTCCACGGACAG	TGG	R	299	318	45	40.48
44	HPV52	E8^E2	GGACACTGCGGAGGTCTTGG	AGG	R	436	455	65	50.83
45	HPV52	E8^E2	GGCACCCACGGACACTGCGG	AGG	R	427	446	75	46.81
46	HPV52	E8^E2	TTTGCGGGGACAACAATCCG	TGG	F	204	223	55	46.54
47	HPV52	E8^E2	GGTCGTCGTCGTTTCTGTGG	TGG	R	386	405	60	44.22
48	HPV53	E8^E2	GGACTCTCTGGCTGTGGTAG	TGG	R	364	383	60	60.09
49	HPV53	E8^E2	GGGACGTTTTCCCGGCCGCA	GGG	R	427	446	70	58.07
50	HPV53	E8^E2	GGAGGTGGTGGGGGTCTTAT	GGG	R	481	500	60	54.72
51	HPV53	E8^E2	GGCTGTGGTAGTGGACTGTG	TGG	R	373	392	60	52.52
52	HPV53	E8^E2	AACAACACCGAAATCGACAG	TGG	F	307	326	45	48.73
53	HPV53	E8^E2	GCACCTGTAGTACATATAAA	AGG	F	340	359	35	47.27
54	HPV53	E8^E2	GGTGCCCACGGGCGTGGAGG	TGG	R	466	485	80	44.75
55	HPV53	E8^E2	TGTGGTAGTGGACTGTGTGG	TGG	R	376	395	55	43.15
56	HPV53	E8^E2	GTAGGTGCCCACGGGCGTGG	AGG	R	463	482	75	41.17
57	HPV56	E8^E2	GGACGTGGAGGTGGTGGTGG	TGG	R	433	452	70	56.67
58	HPV56	E8^E2	GGTGGTGGTGGTGGTCTTGT	GGG	R	442	461	60	42.36
59	HPV58	E8^E2	ACTGTAATATGTCATCCACA	TGG	F	353	372	35	63.81
60	HPV58	E8^E2	CCAACAACGAAAGTACACAG	GGG	F	92	111	45	59.61
61	HPV58	E8^E2	GCCGTGGACAGTAGACCACG	AGG	F	187	206	65	51.17
62	HPV58	E8^E2	TGCTGACCCAAAGACCACCG	AGG	F	66	85	60	50
63	HPV58	E8^E2	AAAGTATACAGACTGCGCCG	TGG	F	171	190	50	47.37
64	HPV58	E8^E2	TGTTACTGTAACATACACAA	CGG	F	414	433	30	44.84
65	HPV58	E8^E2	CTAACTGTACATACAAAGGG	CGG	F	230	249	40	42.73
66	HPV58	E8^E2	ATCACTGGTCCAATGCCATG	TGG	R	127	146	50	40.54
67	HPV59	E8^E2	GGAAAGCGACCAAGACAGTG	TGG	F	154	173	55	50.37
68	HPV59	E8^E2	ATAGGCGTAGTGTTACTGCA	AGG	R	236	255	45	47.03
69	HPV59	E8^E2	TTGCTCAGAAGATCCAGCAG	TGG	R	454	473	50	43.68
70	HPV59	E8^E2	ATATTTCCTCTACCTGGCAT	TGG	F	374	393	40	42.81
71	HPV59	E8^E2	AATAGTGTACAAATACATGT	TGG	F	493	512	25	42.15
72	HPV66	E8^E2	TGTGGGCGCCCAAGACGCCG	CGG	F	120	139	75	51.41
73	HPV66	E8^E2	TATATAAAGATGAAACACAA	CGG	F	452	471	20	50.22
74	HPV66	E8^E2	TCCTGGTCTGTGGGATACCG	CGG	R	388	407	60	47.42
75	HPV66	E8^E2	GGCGTCTTGGGCGCCCACAA	AGG	R	409	428	70	41.85
76	HPV66	E8^E2	TGGCTTTGTGTGTTGATACG	TGG	R	260	279	45	41.14
77	HPV68	E8^E2	GGACACGTTGTCGGGCTCAG	AGG	R	322	341	65	49.42
78	HPV68	E8^E2	GGTGCATGGGGCACCTGCGG	TGG	R	403	422	75	47.65
79	HPV68	E8^E2	TGAGCCCGACAACGTGTCCG	TGG	F	186	205	65	42.42
80	HPV68	E8^E2	TGGGAGGTCGACACCGTCCA	CGG	R	301	320	65	41.86
81	HPV73	E8^E2	TTAAAAATGTAACAACAACA	TGG	F	323	342	20	54.99
82	HPV73	E8^E2	GGTGTCAACGTTGGTGCAGG	TGG	R	361	380	60	53.45
83	HPV73	E8^E2	GGTGGTGGTGTTGGTGGTTG	TGG	R	382	401	60	50.36
84	HPV73	E8^E2	GGTGCAGGTGGTGGTGGTGT	TGG	R	373	392	65	42.12
85	HPV73	E8^E2	AGGTTATGCAAACAATCCAG	GGG	R	290	309	40	41.34
86	HPV82	E8^E2	GCAGGTGTCCACGGACAGCG	GGG	R	301	320	70	66.8
87	HPV82	E8^E2	GGGTGCTGTAGGTACTGTAA	GGG	R	468	487	50	65.45
88	HPV82	E8^E2	GGAATAGGTATTGGTCGTGG	GGG	R	406	425	50	57.11
89	HPV82	E8^E2	GGGGTGCTGTAGGTACTGTA	AGG	R	467	486	55	55.84
90	HPV82	E8^E2	CTGCAGGCCCAAACACCGGA	GGG	F	245	264	65	46.18
91	HPV82	E8^E2	GGACAGCGGGGTGACTGTGG	AGG	R	313	332	70	46.14
92	HPV82	E8^E2	GGGGTGACTGTGGAGGAGTC	GGG	R	320	339	65	44.55
93	HPV82	E8^E2	GGCGGAATAGGTATTGGTCG	TGG	R	403	422	55	44.05
94	HPV82	E8^E2	GGCTTCTGTGGTGCCCGCGG	AGG	R	382	401	75	43.72
95	HPV82	E8^E2	GGAACTGCAGGCCCAAACAC	CGG	F	241	260	60	41.43